Simple and inexpensive incorporation of 19F-tryptophan for protein NMR spectroscopy.
نویسندگان
چکیده
Fluorine-containing amino acids are valuable probes for the biophysical characterization of proteins. Current methods for (19)F-labeled protein production involve time-consuming genetic manipulation, compromised expression systems and expensive reagents. We show that Escherichia coli BL21, the workhorse of protein production, can utilise fluoroindole for the biosynthesis of proteins containing (19)F-tryptophan.
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عنوان ژورنال:
- Chemical communications
دوره 48 86 شماره
صفحات -
تاریخ انتشار 2012